Effect of ultraviolet radiation on cell division and microtubule organization inPetunia hybrida protoplasts |
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Authors: | Irina Staxén Catherine Bergounioux Janet F Bornman |
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Institution: | (1) Department of Plant Physiology, University of Lund, Box 7007, S-22007 Lund, Sweden;(2) Laboratoirie de Physiologie Végétale Moleculaire, CNRS URA 1128, Université de Paris XI, Orsay |
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Abstract: | Summary Mesophyll protoplasts isolated fromPetunia hybrida were subjected to UV radiation (280–360 nm) in an attempt to assess whether (a) UV radiation has an effect on cortical microtubule organization, (b) UV radiation affects the progression of protoplasts through the cell cycle, and (c) there is a connection between the effect of UV radiation on cell division and the polymerization state of the microtubules. The proto plasts were irradiated with the following UV doses: 4, 8, 12, and 24mmol photons/m2, 30 min after isolation. Cell cycle analysis and immuno-localization of microtubules were carried out 0, 24, 48, and 72 h after irradiation. The length of cortical microtubules was determined after irradiation and in corresponding controls. We found that UV radiation induced breaks in cortical microtubules resulting in shorter fragments with increasing dose. Also, the protoplasts were delayed in their progression through the cell cycle, with G1 and G2 phases being affected as well as the S phase. The commencement of DNA synthesis in the irradiated protoplasts followed the re-establishment of a microtubule network. At 48 h after irradiation the protoplasts in all treatments, except for the 24 mmol/m2, had cortical microtubules of similar length, and at 72 h after irradiation only the protoplasts that had received 24 mmol photons/m2 had not started dividing.Abbreviations BSA
bovine serum albumin
- DMSO
dimethyl sulfoxide
- FDA
fluorescein diacetate
- MT
microtubules
- MTSB
microtubule stabilizing buffer
- PAR
photosynthetically active radiation (400–700 nm)
- PBS
phosphate buffered saline
- UV
ultraviolet |
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Keywords: | Cell cycle Flow cytometry Microtubules Petunia hybrida Protoplasts UV radiation |
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