Distribution and partial characterisation of immunoreactivity to the putative C-terminus of rat pancreastatin |
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Authors: | William J Curry Colin F Johnston Christopher Shaw Keith D Buchanan |
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Institution: | Department of Medicine, The Queen's University of Belfast, Belfast, N. Ireland |
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Abstract: | The presumptive C-terminal nonapeptide of rat pancreastatin was synthesised based upon the sequence of rat chromogranin A (CGA) analogous to that of porcine pancreastatin as contained within porcine CGA. Antisera were produced which were used to determine the qualitative and quantitative distribution of pancreastatin-like immunoreactivity in rat tissues by immunocytochemistry and radioimmunoassay respectively. Pancreastatin-like immunoreactivity was most abundant in pituitary, adrenal, gastric corpus and thyroid with considerably lower levels detected in the remainder of the gastroentero-pancreatic system and brain. Immunoreactivity was localised exclusively in endocrine cells and the relative abundance of immunoreactive cells paralleled the levels obtained radioimmunometrically. Chromatographic characterisation of pancreastatin-like immunoreactivity revealed molecular heterogeneity. Immunoreactive peptides of similar size to synthetic rat pancreastatin were present in gastrointestinal tissues and thyroid. These data indicate a tissue specific processing of CGA in the rat. |
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Keywords: | Pancreastatin Chromogranin A Immunocytochemistry Radioimmunoassay Gel permeation chromatography HPLC |
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