霍乱弧菌脂多糖O抗原基因在大肠杆菌中的克隆及表达

经典生物型及埃尔托生物型霍乱弧菌的染色体DNA片段分别与载体质粒pUC18,B.S(M13~+)进行克隆,从克隆株中筛选到能表达霍乱弧菌脂多糖O抗原基因的重组子。它们所表达的脂多糖O抗原具有很好的抗原性及免疫原性,其重组质粒pMG-301、pMG-302经酶切分析表明,外源片段大小分别为8.4kb,7.6kb,较文献报道的16kb要小,而且基因结构之间也存在很大差异。

Molecular Cloning and Expression of Vibrio cholerae LPS O-Antigen Genes in E. coli HB101

The chromosomal fragments of Vibrio Cholerae Classic Biotype and Eltor Biotype were cloned respectively with vetor plasmid pUC18 and B.S(M13+). We got recombinants which could express the LPS O-Antigen of Vibrio cholerae, and the expressed O-Antigen expressed had very good specificity and immunogenecity. After analyzing the recombinant plasmids by restriction endonucleases digestion, we found the size of foreign fragments in pMG-301 and pMG-302 were 8.4kb and 7.6kb, which were much smaller than 16kb fragment reported by documents and great difference existed between the gene's structure.