Free radical mediated interaction of ascorbic acid and ascorbate/Cu(II) with viral and plasmid DNAs |
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Authors: | Günhan Erdem Cihan öner Ahmet M. önal Duygu Kisakürek A. Y. ögüs |
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Affiliation: | (1) Department of Biology, Hacettepe University, Beytepe, 06532 Ankara, Türkiye;(2) Department of Science, Middle East Oechnical University, Ankara, Türkiye;(3) Department of Chemistry, Middle East Oechnical University, Ankara, Türkiye |
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Abstract: | Previous studies indicate that ascorbic acid, when combined with copper or iron cleaves several viral DNA. ln this study, we generated the ascorbate radical anion electrochemically in a simple chemical environment without the participation of a metal ion. This solution possesses viral DNA scission activity. Ohe absence of catalytic metal ions [Fe (III) and Cu(II)] in the incubation medium was evidenced by metal chelating agents such as desferrioxamine and EDTA. Ohe radical quenching at high EDTA concentration was attributed to ionic strength of EDTA rather than metal chelation. Ohe effects of antioxidants, radical scavangers, catalase, superoxide dismutase and some proteins on DNA cleavage have been tested. Cleavage may not arise directly from ascorbate free radical but the reaction of the radical form of ascorbate with oxygen may produce the actual reactive species. Aerobic oxidation of ascorbate itself strictly requires transition metal catalysts, however electrochemically produced ascorbyl radical avoided the kinetic barrier that prevented direct oxidation of ascorbic acid with oxygen and eliminated the need for the transition metal ion catalysts. |
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Keywords: | Ascorbate/Cu(II) ascorbic acid ascorbyl radical DNA damage free radicals λ .-DNA |
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