Direct determination of alginate content in brown algae by near infra-red (NIR) spectroscopy |
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Authors: | Svein Jarle Horn Einar Moen Kjetill Østgaard |
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Institution: | (1) Department of Biotechnology, The Norwegian University of Science and Technology NTNU, N-7491 Trondheim, Norway |
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Abstract: | The methods used to quantify total alginate in brown algal tissue are time-consuming and may also be misleading, so faster
and simpler methods for measuring alginate content would be beneficial in a variety of applications. This study reports on
the use of near infra-red (NIR) analysis to monitor the alginate content of Laminaria hyperborea stipe during biodegradation.
NIR reflectance spectra were recorded for 78 different freeze-dried samples of its stipe. The samples were collected during
several biological degradation experiments and the total alginate content varied from 2.2 to 40.8% Na-alginate (w/w), determined
by established methods based on ion exchange. Data analysis was performed using multivariate calibration methods in order
to relate the spectral data to the alginate content. PLS2 analysis revealed some dependence on material type, probably reflecting
differences in polyphenol content. In the end, a PLS1 model with 9 components was selected. The calculated model was validated
both with internal data and with an external test set. Internal full cross validation explained 96.6% of the variance in alginate
content. The external validation showed that the PLS1 model was able to predict the alginate concentration with a root mean
square prediction accuracy of 2.1%.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | NIR brown algae alginate Laminaria hyperborea |
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