Isolation, purification and characterization of levorin produced by Streptomyces levoris 99/23 |
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Authors: | Lubka Kozhuharova Velizar Gochev Lidia Koleva |
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Institution: | (1) University of Food Technologies, 26 Maritza Blvd, 4002 Plovdiv, Bulgaria |
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Abstract: | Levorin produced by Streptomyces levoris 99/23 was isolated, purified and characterized. It was established that 80% of the levorin was localized in the mycelium
and only 20% was in the cell-free supernatant. Amorphous yellow levorin with activity of 24 000 IU/mg and 96% purity was obtained.
The preparation exhibited three absorption maxima: at λ 362, 382 and 404 nm. The antibiotic contained seven components: A0, A1, A2, A3, A4, and two unidentified ones. According to its composition, the preparation corresponded to the levorin used for medicinal
purposes. However, the levorin produced by S. levoris 99/23 contained half as much levorin A2 and a more than 100 times larger quantity of the more active and less toxic component levorin A3. |
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Keywords: | Streptomyces levoris Levorin Biosynthesis Isolation Purification Composition |
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