Carboxypeptidases of germinating triticale grains |
| |
Authors: | Drzymala Adam Prabucka Beata Bielawski Wiesław |
| |
Institution: | (1) Department of Biochemistry, Warsaw Agricultural University, Nowoursynowska 159, 02-776 Warszawa, Poland |
| |
Abstract: | Presence of five carboxypeptidases was found in endosperm of germinating triticale grains, while two of them in scutellum.
Changes of their activities during four days of germination suggest that carboxypeptidase II plays an important role at initial
stage of germination, while carboxypeptidases I and III - at subsequent stages of the process. High activity of carboxypeptidase
II both in scutellum and endosperm of dry grains accompanied by its decrease during germination, and on the other hand, the
appearance of carboxypeptidases I and III activities at the 2nd and 3rd day of the process seems to confirm such functions of these enzymes. Experiments with GA3 indicated that carboxypeptidase I was synthesized in scutellum, and carboxypeptidase III — in aleurone layer. Carboxypeptidases
I and II cleave N-CBZ-Phe-Ala, and carboxypeptidase III — N-CBZ-Ala-Met and N-CBZ-Ala-Phe as substrates with the highest rate. |
| |
Keywords: | carboxypeptidase triticale germination GA3 |
本文献已被 SpringerLink 等数据库收录! |
|