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Mutagenicity of ptaquiloside, the carcinogen in bracken, and its related illudane-type sesquiterpenes II. Chromosomal aberration tests with cultured mammalian cells |
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| Authors: | Atsuko Matsuoka Akiko Hirosawa Shinasku Natori Shigeo Iwasaki Toshio Sofuni Motoi Ishidate Jr. |
| Affiliation: | a Division of Mutagenesis, National Institute of Hygienic Sciences, Kamigoya, Setagaya-ku, Tokyo 158, Japan b Meiji Colleger of Pharmacy, Yato-cho, Tanashi-shi, Tokyo, Japan c Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan |
| Abstract: | The chromosomal aberration test using a Chinese hamster lung cell line (CHL) was carried out on ptaquiloside and its related compounds, hypoloside B, hypoloside C, illudin M and illudin S. Ptaquiloside induced chromosomal aberrations at doses as low as 4.5 μg/ml (0.0113 mM). The clastogenic effect was ph-dependent. The same activity was observed at a 90-fold higher dose at pH 5.3 in the culture medium compared with the activity at pH 74. or pH 8.0. Both hypoloside B and hypoloside C were also clastogenic at almost the same dose levels as that of ptaquiloside. Illudin M and illudin S were also potet clastogens and induced aberrations at much lower doses than ptaquiloside. These results suggest that the clastogenic effect is involved in the mechanism of carcinogenic potency of ptaquiloside in animals. |
| Keywords: | Chromosomal aberration test Chinese hamster lung cells Ptaquiloside Hypoloside B Hypoloside C Illudin M Illudin S |
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