Potato transformation byAgrobacterium rhizogenes Ri plasmid |
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Authors: | M. Ondřej M. Hrouda P. Kostřica |
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Affiliation: | 1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, ?eské Budějovice, Na s ádkách 702, CS-370 05 2. Institute of Potato Research and Breeding, Havlí?k?v Brod, Czechoslovakia
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Abstract: | Potato root cultures transformed bypRiA4 T-DNA do not regenerate plants in vitro spontaneously, but regeneration can be induced by a sequence of callogenesis and regeneration media. Both Tl and Tr-DNA were found by Southern blotting. Regenerated transformants showed characteristic morphological deviations in the invitro cultures. Plants in the soil showed differences in the inflorescence and tuber development. |
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