Immunoassay method to check the flagellin mediated binding of <Emphasis Type="Italic">Stenotrophomonas maltophilia</Emphasis> to polystyrene |
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Authors: | A K Zgair S Chhibber |
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Institution: | 1.Department of Microbiology,Panjab University,Chandigarh,India |
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Abstract: | Plate count and spectrophotometric methods have been used to asses the ability of an organism to attach to different surfaces
and form biofilms. In the present study we report a highly sensitive, specific and quick method to check the role of flagellin
in bacterial adhesion to polystyrene. Flagellin from Stenotrophomonas maltophilia showed high affinity for polystyrene (P < 0.05), which decreased on pretreatment of flagellin with anti-flagellin in a dilution dependent manner. In an enzyme immunoassay
format a positive correlation was detected between the anti-flagellin dilutions and flagellin attachment to polystyrene (correlation
coefficient +0.860155). These evidences conclusively prove the involvement of flagella in the adhesion of S. maltophilia to polystyrene surface and enzyme immunoassay, a quick and reliable method to check this phenomenon. |
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