Molecular analysis and mapping of two genes encoding maize glutathione S-transferases (GST I and GST II) |
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Authors: | L Rossini M E Pè C Frova K Hein and M Sari-Gorla |
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Institution: | (1) Department of Genetics and Microbiology, University of Milan, Via Celoria 26, 20133 Milan, Italy |
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Abstract: | Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which
(Gst I, II andIII) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism
including a “null” variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA
from “null” and “plus” genotypes, usingGst I- andGst II-specific probes, indicated that theGst I gene controls the expression of the two major GST isoforms expressed in roots.Gst I andGst II were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized.Gst I was localized on the long arm of chromosome 8, while two putativeGst II loci were mapped to chromosomes 8 (70 cM fromGst I) and 10, respectively. |
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Keywords: | Glutathione S-transferase Maize Gene expression Mapping |
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