| 马铃薯Alfin1-like锌指蛋白基因克隆与序列分析 |
| |
| 引用本文： | 刘瑞娟,王海庆.马铃薯Alfin1-like锌指蛋白基因克隆与序列分析[J].西北植物学报,2007,27(4):657-661. |
| |
| 作者姓名： | 刘瑞娟 王海庆 |
| |
| 作者单位： | 1. 中国科学院西北高原生物研究所,西宁,810001;中国科学院研究生院,北京,100049
2. 中国科学院西北高原生物研究所,西宁,810001 |
| |
| 基金项目： | 中国科学院"西部之光"人才培养计划 |
| |
| 摘 要： | 利用紫花苜蓿盐胁迫相关锌指结构蛋白Alfin1基因cDNA序列,通过电子克隆在GenBank中对马铃薯同源EST序列进行查询比较和拼接,获得了1个含有完整编码区的cDNA序列,并通过RT-PCR成功获得了该序列.获得的全长cDNA序列中包含1个747 bp的最大读码框,编码248个氨基酸,将其命名为Stfin1.氨基酸序列分析表明存在典型的Cys4-His-Cys3锌指结构,与Alfin1一致性达93.09%.从结构分析结果推测Stfin1与Alfin1在功能上具有一定的相关性.
|
| 关 键 词： | 锌指蛋白 电子克隆 马铃薯 |
| 文章编号： | 1000-4025（2007）04-0657-05 |
| 修稿时间： | 2006-11-09 |
Cloning and Sequencing of an Alfin1-like Zinc Finger Protein Gene in Solanum tuberosum |
| |
| LIU Rui-juan,WANG Hai-qing.Cloning and Sequencing of an Alfin1-like Zinc Finger Protein Gene in Solanum tuberosum[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(4):657-661. |
| |
| Authors: | LIU Rui-juan WANG Hai-qing |
| |
| Institution: | 1. Northwest Plateau Institute of Biology,the Chinese Academy of Sciences, Xining 810001 ,China; 2 .Graduate School of Chinese Academy of Sciences,Beijing 100049,China |
| |
| Abstract: | A full length cDNA sequence was obtained by in silico cloning.After searching against potato EST database in GenBank using Alfin1 cDNA sequence from alfalfa.The native cDNA sequence denoted as Stfin1(Solanum tuberosum Alfin1-like protein1) was isolated with RT-PCR amplification.Stfin1 contains a maximum open reading frame of 747 bp encoding a peptide with 248 amino acids and a stop codon.The Stfin1-deduced amino acid sequence showed 93.09% identity and shared a conserved typical Cys4-His-Cys3 zinc finger motif with Alfin1.Its sequence conservation and structure similarity with Alfin1 implied the functional correlation between Stfin1 and Alfin1 in development processes and responses to abiotic stresses. |
| |
| Keywords: | zinc finger protein in silico cloning Solanum tuberosum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北植物学报》浏览原始摘要信息 |
| 点击此处可从《西北植物学报》下载免费的PDF全文 |
