An FMRFamide antiserum differentiates between populations of antigens in the brain and retrocerebral complex of the locust,Schistocerca gregaria |
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Authors: | Camilla M. Myers Dr. Peter D. Evans |
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Affiliation: | (1) A.F.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Cambridge, UK;(2) AFRC, Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, CB2 3EJ Cambridge, UK |
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Abstract: | Summary The distribution of FMRFamide-irmunoreactive cell bodies in the brain and retrocerebral complex of the locust, Schistocerca gregaria, is described. Most of the immunoreactive cell bodies are found in the pars intercerebralis and in the optic lobes. Many, but not all, of the cell bodies also react with an antiserum raised against bovine pancreatic polypeptide, but this antiserum also reveals another population of cells that stain selectively with this antiserum. In addition to the cell bodies, numerous immunoreactive processes are revealed by both antisera in neuropilar regions of the brain. The results of blocking experiments suggest that a differential distribution of three locust antigens can be determined from the examination of alternate serial sections stained with the two antisera used. |
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Keywords: | Nervous system Immunocytochemistry FMRFamide Bovine pancreatic polypeptide Insects Schistocerca gregaria |
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