Immunocytochemical identification of laticifers |
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| Authors: | K. J. Wilson B. H. Petersen D. D. Biesboer |
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| Affiliation: | (1) Department of Biology, Purdue University School of Science, 1125 E. 38th St., 46223 Indianapolis, IN, USA;(2) Lilly Laboratories for Clinical Research, Eli Lilly & Co., USA;(3) Department of Botany, University of Minnesota, USA |
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| Abstract: | Summary sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex ofAsclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos ofA. syriaca andStapelia bella and embryos ofA. tuberosa. Laticifers did not fluoresce in shoots ofA. tuberosa andEuphorbia tirucalli, in embryos ofE. marginata, or in petioles ofMusa paradisiaca andCichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence). |
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| Keywords: | Immunocytochemistry Non-articulated laticifers Articulated laticifers Asclepiadaceae |
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