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Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I
Authors:Lee Soo-Ung  Chun Ha-Chung  Huh Sun
Affiliation:1Department of Environmental and Tropical Medicine, School of Medicine, KonKuk University, Chungju, 380-701, Korea.;2Department of Radiation Oncology, College of Medicine, Hanyang University, Seoul 133-731, Korea.;3Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
Abstract:The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.
Keywords:Platyhelminthes   molecular phylogeny   28S ribosomal DNA   mitochondrial cytochrome c oxidase subunit 1
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