Angiotensin-converting enzyme inhibition and food restriction in diabetic mice do not correct the increased sensitivity for ischemia-reperfusion injury |
| |
Authors: | Gerry Van der Mieren Ines Nevelsteen Annelies Vanderper Wouter Oosterlinck Willem Flameng Paul Herijgers |
| |
Institution: | 1. Laborat??rio de Experimenta??o Animal e Laborat??rio de Cardiologia Molecular e Celular, Instituto de Cardiologia/Funda??o, Universit??ria de Cardiologia, Av. Princesa Isabel, 395, Bairro Santana, Porto Alegre, RS, 90620 001, Brazil 2. Departamento de Fisiologia e Biof??sica do Instituto de Ci??ncias Biom??dicas, Universidade de S?o Paulo, S?o Paulo, Brazil 3. Servi?o de Endocrinologia, Hospital de Cl??nicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
|
| |
Abstract: | Background Metabolic syndrome is characterized by insulin resistance, which is closely related to GLUT4 content in insulin-sensitive tissues. Thus, we evaluated the GLUT4 expression, insulin resistance and inflammation, characteristics of the metabolic syndrome, in an experimental model. Methods Spontaneously hypertensive neonate rats (18/group) were treated with monosodium glutamate (MetS) during 9 days, and compared with Wistar-Kyoto (C) and saline-treated SHR (H). Blood pressure (BP) and lipid levels, C-reactive protein (CRP), interleukin 6 (IL-6), TNF-?? and adiponectin were evaluated. GLUT4 protein was analysed in the heart, white adipose tissue and gastrocnemius. Studies were performed at 3 (3-mo), 6 (6-mo) and 9 (9-mo) months of age. Results MetS rats were more insulin resistant (p<0.001, all ages) and had higher BP (3-mo: p<0.001, 6-mo: p?=?0.001, 9-mo: p?=?0.015) as compared to C. At 6 months, CRP, IL-6 and TNF-?? were higher (p<0.001, all comparisons) in MetS rats vs H, but adiponectin was lower in MetS at 9 months (MetS: 32?±?2, H: 42?±?2, C: 45?±?2 pg/mL; p<0.001). GLUT4 protein was reduced in MetS as compared to C rats at 3, 6 and 9-mo, respectively (Heart: 54%, 50% and 57%; Gastrocnemius: 37%, 56% and 50%; Adipose tissue: 69%, 61% and 69%). Conclusions MSG-treated SHR presented all metabolic syndrome characteristics, as well as reduced GLUT4 content, which must play a key role in the impaired glycemic homeostasis of the metabolic syndrome. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|