Intracellular pH controls the development of new potassium conductance after fertilization of the sea urchin egg |
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Authors: | Sheldon S Shen Richard A Steinhardt |
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Institution: | Department of Zoology, University of California, Berkeley, CA 94720, USA |
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Abstract: | Fertilization of the sea urchin egg leads to a sequence of changes at the egg surface and the interior cytoplasm. Among these changes are the transient elevation of internal calcium levels, alkalization of the cytoplasm and development of new K+-conductance. In the series of experiments reported here, we separate the effects on potassium activation of the calcium release and the rise in the intracellular pH. The development of new K+-conductance was dependent on alkalization of the egg cytoplasm, and not on a rise of internal calcium levels. The effects of 2,4-dinitrophenol, N-ethylmalemide, antimycin A and oligomycin suggest that the maintenance of the alkaline internal pH of fertilized eggs appears to be dependent on membrane ATPase activity. |
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