In vitro assembly of microtubule proteins from myxamoebae of Physarum polycephalum |
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Authors: | Anne Roobol Christopher I. Pogson Keith Gull |
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Affiliation: | Biological Laboratory, University of Kent, Canterbury, Kent, CT2 7NJ, UK |
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Abstract: | Microtubule protein of >95% purity has been isolated by self-assembly from concentrated cell extracts of myxamoebae of Physarum polycephalum. Ninety-eight percent of the amoebal microtubule protein was tubulin. Both a and β subunits of amoebal tubulin were different from neurotubulin α and β subunits, but very similar to those of Tetrahymena ciliary tubulin. The non-tubulin components, which co-purified with tubulin through three assembly cycles, were essential to microtubule formation and contained several polypeptides including some of apparent molecular weights 49000, 57000 and 59000. Purified amoebal microtubule protein formed microtubules on warming in the absence of glycerol which were cold- and Ca2+-labile. In vitro, microtubule assembly was inhibited by vinblastine, benzimidazole derivatives and griseofulvin, but not by 10?4 M colchicine. Amoebal tubulin had a much lower affinity than neurotubulin for colchicine. |
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