Toward a multicolor chromosome bar code for the entire human karyotype by fluorescence in situ hybridization |
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Authors: | S Müller M Rocchi M A Ferguson-Smith J Wienberg |
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Institution: | (1) Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK Tel.: +44-1223-333725; Fax: +44-1223-333346; e-mail: jw@mole.bio.cam.ac.uk, GB;(2) Istituto di Genetica, University of Bari, I-70126 Bari, Italy, IT |
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Abstract: | A colored banding pattern for human chromosomes is described that distinguishes each chromosome in a single fluorescence
in situ hybridization with a set of subregional DNA probes. Alu/polymerase chain reaction products of various human/rodent
somatic cell hybrids (fragment hybrids) were pooled into two probe sets that were labeled differentially and detected by red
and green fluorescence. Chromosome regions hybridized by DNA present in both pools appeared yellow. The result was a multi-color
set of 110 distinct signals per haploid chromosome set for the human karyotype. Each individual chromosome showed a unique
sequence of signals, a result termed the “chromosome bar code”. The reproducibility of the hybridization pattern in various
labeling and hybridization experiments was analyzed by computer densitometry. We have applied the chromosome bar code both
in diagnostic cytogenetics and in genome studies. The approach allows the rapid identification of chromosomes and chromosome
rearrangements. Although not yet showing the resolution of classical banding patterns, the present experiments demonstrate
various applications in which the present multi-color bar code can significantly add to the spectrum of cytogenetic techniques.
Received: 18 December 1996 / Accepted: 10 February 1997 |
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