UDP-glucose:isoflavone 7-O-glucosyltransferase from roots of chick pea (Cicer arietinum L.) |
| |
Authors: | Johannes Köster Wolfgang Barz |
| |
Institution: | Lehrstuhl für Biochemie der Pflanzen, Universität Münster, D-4400 Münster, West Germany |
| |
Abstract: | A glucosyltransferase which catalyzes the glucosylation of isoflavones in position 7 using uridine diphosphate glucose as glucosyl donor has been purified about 120-fold from 4-day-old roots of chick pea (Cicer arietinum L.). The soluble enzyme showed a pH optimum of 8.5–9.0 and a molecular weight of 50,000. The Km for uridine diphosphate glucose was 200 μm and for the isoflavones biochanin A and formononetin, 12 and 24 μm, respectively. While the aforementioned 4′-methoxy isoflavones were the best substrates, the 4′-hydroxy isoflavones genistein and daidzein were poor substrates. The enzyme was unable to catalyze the glucosylation of hydroxy substitutes isoflavanones, flavones, flavanones, flavonols, coumarins, cinnamic acids, and benzoic acids. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|