Mitogenic signaling mediated by oxidants in retinol treated sertoli cells |
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Authors: | Felipe Dal-Pizzol F bio Klamt Rodrigo J. S. Dalmolin Elena A. Bernard Jos Cl udio F. Moreira |
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Affiliation: | Departamento de Bioquímica, ICBS-Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. pizzol@ez-poa.com.br |
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Abstract: | Recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer. Recently the causal mechanism has begun to be clarified. We report here that retinol-induced oxidative stress is accompanied by cellular proliferation. Retinol (7 μM) significantly induced thiobarbituric acid reactive species (TBARS) formation, which was inhibited by trolox, superoxide dismutase, N-acetylcysteine and ethanol. This was accompanied by an increase in DNA synthesis and focus formation in cultured rat Sertoli cells. Antioxidants and ethanol inhibited retinol-induced DNA synthesis. Our findings suggest that retinol-induced oxidative stress was associated with cellular proliferation complementing our understanding of the significance of retinol supplementation in neoplastic transformation. |
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