Production of L- AND D-lactate by Pediococcus pentosaceus in batch and steady-state continuous culture |
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Authors: | Margareta H Häggström E Andersson |
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Institution: | Department of Technical Microbiology, University of Lund, Lund, Sweden |
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Abstract: | The fast growth and acid production of a strain of Pediococcus pentosaceus , used as a starter culture in the production of dry sausages, was dependent on the presence of acetate. In a batch culture on a mixture of glucose and sucrose both sugars were consumed simultaneously. Similar growth rates and product yields were obtained on glucose and sucrose, d - AND l -lactate were produced via a D- and L-lactate dehydrogenase (LDH), respectively, and no racemase was present. In batch cultures about 15% of the lactic acid produced was the D-isomer, whereas in a sucrose-limited, continuous culture the fraction of D-lactic acid increased with decreasing dilution rate. The results are discussed in relation to the two LDH activities. |
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