Emergence and development of resistance to antimicrobial chemicals and heat in spores of Bacillus subtilis |
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Authors: | S.P. Gorman E.M. Scott E.P. Hutchinson |
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Affiliation: | Department of Pharmacy, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland |
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Abstract: | The emergence and development of chemical and thermal resistance in spores of Bacillus subtilis was examined. The chemicals studied were of the disinfectant type: glutaraldehyde, hypochlorite, hypochlorite-methanol and povidone-iodine. Growth and sporulation were followed by electron microscopy and resistance assigned to specific stages in relation to 45Ca and DPA accumulation. A sequential development of resistance was observed with thermal resistance appearing first at early Stage V corresponding to maturation of cortex and deposition of rudimentary spore coat material. Chemical resistance coincided with middle to late Stage V dependent on the chemical concerned. A progressive development of resistance was observed on prolonged incubation in sporulation medium and was affected by inclusion of lysozyme in the spore washing sequence. |
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