|
|||||
|
|
The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase |
|
| Authors: | Bert Overduin Saskia A Hogenhout Erik A van der Biezen Michel A Haring H John J Nijkamp and Jacques Hille |
| Institution: | (1) Department of Genetics, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1081 Amsterdam, HV, The Netherlands;(2) Present address: Universiteit van Amsterdam, Section Plant Physiology, Kruislaan 318, 1098 Amsterdam, SM, The Netherlands |
| Abstract: | The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (AC Tase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus. |
| Keywords: | Tomato Asc locus aspartate carbamoyltransferase PCR RFLP linkage analysis |
| 本文献已被 SpringerLink 等数据库收录！ | |