| 5种药用石韦总黄酮的提取及测定 |
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| 引用本文： | 李晓丹,肖娅萍. 5种药用石韦总黄酮的提取及测定[J]. 中国野生植物资源, 2009, 28(1): 59-61 |
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| 作者姓名： | 李晓丹 肖娅萍 |
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| 作者单位： | 1. 内蒙古医学院,基础医学院,内蒙古,呼和浩特,010050
2. 陕西师范大学生命科学学院,陕西,西安,710062 |
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| 基金项目： | 陕西米仓山自然保护区综合科学考察项目 |
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| 摘 要： | 采用正交实验设计探究石韦总黄酮的提取工艺，并用该工艺提取了5种药用石韦总黄酮，采用分光光度法测定总黄酮含量。旨在为石韦药材的黄酮提取及石韦药材质量控制和进一步有效利用提供参考。研究结果表明，最佳提取方案为：55％乙醇，回流提取3次，每次2h。测得毡毛石韦的总黄酮含量明显较高，而华北石韦总黄酮含量最低，不同种石韦总黄酮含量存在显著差异。
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| 关 键 词： | 药用石韦 总黄酮 评价指标 |
Determination of Total Flavonoids from 5 Species of Pyrrosia Leaf |
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| Li Xiaodan,Xiao Yaping. Determination of Total Flavonoids from 5 Species of Pyrrosia Leaf[J]. Chinese Wild Plant Resources, 2009, 28(1): 59-61 |
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| Authors: | Li Xiaodan Xiao Yaping |
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| Affiliation: | Li Xiaodan , Xiao Yapping (1. School of Basic Medical Sciences, Inner Mongolia Medical College, Hohhot 010050, China; 2. College of Life Science, Shaanxi Normal University, Xi＇an 710062, China) |
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| Abstract: | The optimum extraction condition for total flavonoids was investigated by orthogonal design. Using this method, the total flavonoids from 5 different Pyrrosia leaves were extracted and determined by UV spectrophotometer. The objective is to provide reference for extraction of flavonoids and quality con- trol of Pyrrosia materials. The best condition is 55% ethanol, 2 hours, 3 times. The result showed fla- vonoids content of Pyrrosia drakeana （Franch.） Ching is obviously high whereas flavonoids content of Pyrrosia davidii （ Bak. ） Ching is the lowest. Flavonoids content of different species has large distance. |
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| Keywords: | Pyrrosia leaf total flavonoids evaluated standard |
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