Effect of phospholipase-D on rat kidney mitochondria |
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Authors: | S N A Zaidi A C Shipstone N K Garg |
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Institution: | (1) Division of Biochemistry, Central Drug Research Institute, 226 001 Lucknow |
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Abstract: | Incubation of purified rat kidney mitochondrial fraction with phospholipase-D resulted in the accumulation of phosphatidic
acid in the membrane due to the degradation of membrane-bound phosphatidylcholine, -serine and-ethanolamine Simultaneously
with the hydrolysis of the phospholipids, cholesterol and protein were released from the mitochondrial membrane into the medium,
and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated mitochondrial fraction exhibited increased swellingin vitro in the early stages of incubation (15 min) after which the mitochondria were ruptured. Membrane-bound adenosine triphosphatase
was partially inactivated and the enzyme activity was not significantly restored by incubation with sonicated dispersions
of phosphatidylcholine,-serine and cholesterol. These results indicate that removal of choline, serine and ethanolamine from
membrane-bound phospholipids disrupt phospholipid-cholesterol and phospholipid-protein association and affect functions of
the membrane.
Communication no, 2468. |
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Keywords: | Mitochondria phospholipase-D phosphatidylcholine phosphatidylethanolamine phosphatidylserine swelling adenosine triphosphatase Ca2+ binding |
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