Molecular mapping of <Emphasis Type="Italic">Thinopyrum</Emphasis>-derived Fusarium head blight resistance in common wheat |
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Authors: | Xiaorong Shen Herbert Ohm |
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Institution: | (1) Department of Agronomy, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA |
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Abstract: | Resistance to Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe in wheat (Triticum aestivum L.) was identified in disomic chromosome substitution and translocation lines, into which chromosome 7el2 had been introgressed from wheatgrass, Thinopyrum ponticum. In this study, two chromosome substitution lines with different origins (designated as el1 and el2) and with different reactions to infection by F. graminearum were crossed to develop a segregating mapping population. The objectives of this study were to determine the effectiveness
of this type II resistance and map it on chromosome 7el2. Type II resistance to FHB was characterized in the F2, F2:3 families, F4:5 plants and F5:6 recombinant inbred lines developed by single-seed descent; and the population was characterized in the F2 and F5 with DNA markers along the long arm of 7el. Composite interval mapping revealed a FHB resistance QTL, designated Qfhs.pur-7EL, located in the distal region of the long arm of 7el2 and delimited with flanking markers XBE445653 and Xcfa2240. Additive effects of Qfhs.pur-7EL reduced the number of diseased spikelets per spike following inoculation of one floret in four experiments by 1.5–2.6 and
explained 15.1–32.5% of the phenotypic variation in the populations. Several STS-derived and EST-derived PCR or CAPS markers
were developed in this chromosomal region, and showed the specificity of 7el2 compared to an array of wheat lines possessing other sources of FHB resistance. These markers are useful in an effort to
shorten the chromosome segment of 7el2 and to use for marker-assisted introgression of this resistance into wheat. |
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Keywords: | DNA markers Fusarium head blight QTL mapping Thinopyrum ponticum Wheat (Triticum aestivum) |
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