Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM |
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| Affiliation: | 1. National Bacteriological Laboratory, S-105 Stockholm, Sweden;2. National Defence Research Establishment, FOA-5, S-172 90, Sundbyberg Sweden;3. Institute of Poliomyelitis and Viral Encephalitides, Academy of Medical Sciences, 142782 Moscow, Russia;4. Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium;5. National Institute of Public Health, N-0462 Oslo 4, Norway;6. Aarhus University, 8000, Aarhus, Denmark;7. US Army Medical Research, Institute of Infectious Diseases, Fort Detrick, Frederick 21702 MD USA;8. Umeå Hospital S-901 87 Umeå Sweden;1. Department of Virology, Hôpital Henri Mondor, AP-HP, Université Paris-Est, Créteil, France;2. “Genomics” Platform, Hôpital Henri Mondor, AP-HP, Université Paris-Est, Créteil, France;3. Team “Viruses, Hepatology, Cancer”, INSERM U955, Créteil, France;4. National Reference Center for Viral Hepatitis B, C and Delta, Hôpital Henri Mondor, AP-HP, Créteil, France;5. Department of Hepatology, Hôpital Henri Mondor, AP-HP, Créteil, France;1. Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, 67030-000, Pará, Brazil;2. Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, 21040-900, Rio de Janeiro, Brazil;1. CBGP, INRAE, CIRAD, IRD, Institut Agro, Université de Montpellier, France;2. Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland;3. Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland;4. CBGP, IRD, INRAE, CIRAD, Institut Agro, Université de Montpellier, France;1. Institute of Microbiology and Immunology, Fort Detrick, MD, USA;2. Institute for Biostatistics and Medical Informatics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia, Fort Detrick, MD, USA;3. US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA |
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| Abstract: | IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR.IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies.Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the “one-way” crossing described earlier. |
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