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Cycling of amino-nitrogen between shoots and roots in wheat seedlings
Authors:H. D. Cooper  D. T. Clarkson  M. G. Johnston  J. N. Whiteway  B. C. Loughman
Affiliation:(1) Letcombe Laboratory (now at Long Ashton Research Station, BS18 9AF) Bristol, UK;(2) Department of Agricultural Science, University of Oxford, OX1 3PF Oxford, UK;(3) Animal and Grassland Research Institute, Hurley, SL6 5LR Maidenhead, UK
Abstract:Summary One part of a split root system of wheat seedlings received full nutrient solution with15N-nitrate, the other received an identical solution with unlabelled nitrate. Appearance of labelled amino compounds was measured in the xylem sap exuding from roots not supplied directly with15N-nitrate after removal of the15N-nitrate-fed roots. This material indicates cycling of nitrogen from the shoots and through the roots. About 60 per cent of the nitrogen in the xylem appears to be cycling in this way.
Keywords:Nitrogen cycling  Nitrogen-15 tracer  Split root experiments  Triticum aestivum L.  Wheat  Xylem sap
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