| ColEl-Hhal片断DNA编码产物的体外合成 |
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| 引用本文： | 陈蕙珠. ColEl-Hhal片断DNA编码产物的体外合成[J]. 分子细胞生物学报, 1984, 0(1) |
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| 作者姓名： | 陈蕙珠 |
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| 作者单位： | 中国科学院上海植物生理研究所 |
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| 摘 要： | 在S-30无细胞转录翻译联对系统中,应用小量S-30抽提液,即在每毫升反应混合液内,S-30蛋白质含量从6.6毫克降低至1毫克,不仅对DNA模板具有较高合成能力,而且对降低内源蛋白质合成起了显著作用。以Col El—Hhal的六个片断为模板,在S-30转录翻译联对系统进行蛋白合成,各片段DNA都编码了其相应的蛋白质。体外合成的蛋白产物应用Colicin抗血清的沉淀试验得到验证。
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THE SYNTHESIS OF PROTEIN ENCODED BY COLEI-HHAL FRAGMENT IN A CELL FREE COUPLED TRANSCRIPTION TRANSLATION SYSTEM |
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| CHEN HUI-ZHU. THE SYNTHESIS OF PROTEIN ENCODED BY COLEI-HHAL FRAGMENT IN A CELL FREE COUPLED TRANSCRIPTION TRANSLATION SYSTEM[J]. Journal of Molecular Cell Biology, 1984, 0(1) |
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| Authors: | CHEN HUI-ZHU |
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| Abstract: | In the coupled system for RNAand protein synthesis by using 1mgof S-30 extracts per ml of incubationmixture,it could reduce the backgro-und protein synthesis. Six ColEl-Hhal DNA fragmentswere used as template to direct protein synthesis in lower S-30 extractcouple system. The products were verified by pre-cipitation test with colicin antiserum. |
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| Keywords: | Protein synthesis.ColEl-Hhal fragment.Coupled system. |
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