Employing site-specific recombination for conditional genetic analysis in Sinorhizobium meliloti |
| |
Authors: | Harrison Clarice L Crook Matthew B Peco Gledi Long Sharon R Griffitts Joel S |
| |
Affiliation: | 1Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah 84602;2Department of Biological Sciences, Stanford University, Stanford, California 94305 |
| |
Abstract: | The ability to remove a genetic function from an organism with good temporal resolution is crucial for characterizing essential genes or genes that act in complex developmental programs. The rhizobium-legume symbiosis involves an elaborate two-organism interaction requiring multiple levels of signal exchange. As an important step toward probing rhizobium genetic functions with temporal resolution, we present the development of a conditional gene deletion system in Sinorhizobium meliloti that employs Cre/loxP site-specific recombination. This system enables chemically inducible and irreversible gene deletion or gene upregulation. Recombinase-mediated excision events can be positively or negatively selected or monitored by a colorimetric assay. The system may be adaptable to various bacterial species, in which recombinase activity may be placed under the control of diverse user-defined promoters. This system also shows promise for uses in promoter trapping and biosensing applications. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|