BTI1, an azoreductase with pH-dependent substrate specificity |
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| Authors: | Johansson Hans E Johansson Mary K Wong Albert C Armstrong Eliana S Peterson Erik J Grant Richard E Roy Margaret A Reddington Mark V Cook Ronald M |
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| Institution: | Biosearch Technologies, Inc., 81 Digital Drive, Novato, California 94949-5728 |
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| Abstract: | The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. |
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