Microarray-based evaluation of whole-community genome DNA amplification methods |
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| Authors: | Wang Jian Van Nostrand Joy D Wu Liyou He Zhili Li Guanghe Zhou Jizhong |
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| Affiliation: | 1State Key Joint Laboratory of Environmental Stimulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China;2Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma;3Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 |
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| Abstract: | Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA. |
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