Differently labeled peptide ligands for rapid investigation of receptor expression on a new human glioblastoma cell line |
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Authors: | Fabry M Cabrele C Höcker H Beck-Sickinger A G |
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Affiliation: | a German Wool Research Institute at the University of Technology, Veltmanplatz 8, 52062 Aachen, Germany b Department of Applied Biology, Federal Institute of Technology, 8057 Zürich, Switzerland c Institute of Biochemistry, University of Leipzig, 04103 Leipzig, Germany |
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Abstract: | The neuropeptides vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), and substance P (SP) as well as insulin and insulin-like growth factor 1 (IGF-1) were labeled with biotin, fluorescent dyes, and radioactivity to characterize the expression of peptide receptor of a novel cancer cell line, established from a human glioblastoma multiforme. Thus, not only binding sites could be detected but advantages and disadvantages of the different labels could be compared, too. With all three markers, the presence or absence of the receptors could be answered rapidly and sensitively. The glioblastoma cells express receptors for VIP (IC50 = 9 nM ± 30%), insulin (Kd = 0.66 nM ± 14%, Bmax = 0.028 nM ± 13%), and IGF-1 (Kd = 21 nM ± 25%, Bmax = 1.65 nM ± 24%), but there are no binding sites for NPY and SP. As especially VIP and IGF-1 receptors are expressed in huge amounts, these receptors might be an interesting target for tumor diagnostics and therapy. |
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Keywords: | Biotin and fluorescence labeling Neuropeptides Vasointestinal peptide (VIP) Insulin-like growth factor 1 (IGF-1) Insulin Glioblastoma cells |
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