| Abstract: | The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of l-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methiods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that l-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine. |
