Exon mutations uncouple 5' splice site selection from U1 snRNA pairing |
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Authors: | B Séraphin M Rosbash |
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Affiliation: | Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254. |
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Abstract: | It has previously been shown that a mutation of yeast 5' splice junctions at position 5 (GUAUGU) causes aberrant pre-mRNA cleavages near the correct 5' splice site. We show here that the addition of exon mutations to an aberrant cleavage site region transforms it into a functional 5' splice site both in vivo and in vitro. The aberrant mRNAs are translated in vivo. The results suggest that the highly conserved G at the 5' end of introns is necessary for the second step of splicing. Further analyses indicate that the location of the U1 snRNA-pre-mRNA pairing is not affected by the exon mutations and that the precise 5' splice site is selected independent of this pairing. |
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