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Composition of indolealkylamines of Bufo rubescens cutaneous secretions compared to six other Brazilian bufonids with phylogenetic implications
Authors:Maciel Natan M  Schwartz Carlos Alberto  Rodrigues Pires Júnior Osmindo  Sebben Antonio  Castro Mariana S  Sousa Marcelo V  Fontes Wagner  Ferroni Schwartz Elisabeth N
Affiliation:a Laboratório de Toxinologia, Departamento de Ciências Fisiológicas, Universidade de Brasília, Campus Darcy Ribeiro, Brasília DF 70910-900, Brazil;b Centro Brasileiro de Serviços e Pesquisas em Proteínas, Laboratório de Bioquímica e Química de Proteínas, Departamento de Biologia Celular, Universidade de Brasília, Campus Darcy Ribeiro, Brasília 70910-900, Brazil
Abstract:The composition of indolealkylamines of Bufo rubescens cutaneous secretions was compared to those from six other Brazilian bufonids. Skin, parotoid and tibial gland secretions were obtained for analysis by thin-layer chromatography. A triple-quadrupole mass spectrometer was used to confirm the indolealkylamines standards (serotonin, 5-HT; bufotenin, BTN; dehydrobufotenin, DHB and bufotenidin, BTD). We observed clear variation in the composition of indolealkylamines of the cutaneous secretions studied and also between those found in the skin and parotoid gland secretions of the same species. We discuss the utility of indolealkylamines to the phylogeny of this group of toads.
Keywords:Bufo rubescens   Bufonidae   Indolealkylamines   Parotoid glands   Phylogeny   Skin glands   Skin secretion   Thin-layer chromatography (TLC)
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