Effect of dietary coenzyme Q and fatty acids on the antioxidant status of rat tissues |
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Authors: | Gómez-Díaz C Burón M I Alcaín F J González-Ojeda R González-Reyes J A Bello R I Herman M D Navas P Villalba J M |
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Institution: | (1) Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Córdoba, ES;(2) Laboratorio Andaluz de Biología, Universidad Pablo de Olavide, Sevilla, ES |
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Abstract: | Summary. Wistar rats were fed with different diets with or without supplement coenzyme Q10 (CoQ10) and with oil of different sources (sunflower or virgin olive oil) for six or twelve months. Ubiquinone contents (CoQ9 and CoQ10) were quantified in homogenates of livers and brains from rats fed with the four diets. In the brain, younger rats showed
a 3-fold higher amount of ubiquinone than older ones for all diets. In the liver, however, CoQ10 supplementation increased the amount of CoQ9 and CoQ10 in both total homogenates and plasma membranes. Rats fed with sunflower oil as fat source showed higher amounts of ubiquinone
content than those fed with olive oil, in total liver homogenates, but the total ubiquinone content in plasma membranes was
similar with both fat sources. Older rats showed a higher amount of ubiquinone after diets supplemented with CoQ10. Two ubiquinone-dependent antioxidant enzyme activities were measured. NADH-ferricyanide reductase activity in hepatocyte
plasma membranes was unaltered by ubiquinone accumulation, but this activity increased slightly with age. Both cytosolic and
membrane-bound dicumarol-sensitive NAD(P)H:(quinone acceptor) oxidoreductase (DT-diaphorase, EC 1.6.99.2) activities were
decreased by diets supplemented with CoQ10. Animals fed with olive oil presented lower DT-diaphorase activity than those fed with sunflower oil, suggesting that the
CoQ10 antioxidant protection is strengthened by olive oil as fat source.
Received May 22, 2002; accepted September 20, 2002; published online May 21, 2003
RID="*"
ID="*" Correspondence and reprints: Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba Edificio
Severo Ochoa, Campus de Rabanales, 14071 Córdoba, Spain. |
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Keywords: | : Ubiquinone Quinone reductase Rat liver Plasma membrane Fatty acid Olive oil |
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