Amino acid incorporation by a natural population of Oscillatoria rubescens. A microautoradiographic study |
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Authors: | Gilles Bourdier Jacques Bohatier Mauricette Feuillade Jacques Feuillade |
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Affiliation: | UniversitéBlaise Pascal, Laboratoire de Zoologie Protistologie, Aubière, and Station d'Hydrobiologie Lacustre, 75 avenue de Corzent, Thonon les Bains, France |
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Abstract: | Abstract The incorporation of a very low concentration (0.015 μM) of an [3H]amino acid mixture was measured for a natural population of Oscillatoria rubescens DC in samples from a eutrophic lake, Lake Nantua (France). The kinetics of amino acid incorporation in the size fraction ⩾12 μ m showed that uptake was fast and that the maximum was reached after 4 h. Microautoradiography demonstrated that Oscillatoria rubescens is able to utilize for protein synthesis an external pool of amino acids whose [3H] label becomes distributed generally throughout the cell. |
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Keywords: | Oscillatoria rubescens Cyanobacteria Amino acid incorporation Microautoradiography |
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