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Amino acid incorporation by a natural population of Oscillatoria rubescens. A microautoradiographic study
Authors:Gilles Bourdier  Jacques Bohatier  Mauricette Feuillade  Jacques Feuillade
Affiliation:UniversitéBlaise Pascal, Laboratoire de Zoologie Protistologie, Aubière, and Station d'Hydrobiologie Lacustre, 75 avenue de Corzent, Thonon les Bains, France
Abstract:Abstract The incorporation of a very low concentration (0.015 μM) of an [3H]amino acid mixture was measured for a natural population of Oscillatoria rubescens DC in samples from a eutrophic lake, Lake Nantua (France).
The kinetics of amino acid incorporation in the size fraction ⩾12 μ m showed that uptake was fast and that the maximum was reached after 4 h.
Microautoradiography demonstrated that Oscillatoria rubescens is able to utilize for protein synthesis an external pool of amino acids whose [3H] label becomes distributed generally throughout the cell.
Keywords:Oscillatoria rubescens    Cyanobacteria    Amino acid incorporation    Microautoradiography
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