Piezoelectric urea biosensor based on immobilization of urease onto nanoporous alumina membranes |
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Authors: | Yang Zhengpeng Si Shihui Dai Hongjuan Zhang Chunjing |
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Affiliation: | aCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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Abstract: | The urease was immobilized onto nanoporous alumina membranes prepared by the two-step anodization method, and a novel piezoelectric urea sensing system with separated porous alumina/urease electrode has been developed through measuring the conductivity change of immobilized urease/urea reaction. The process of urease immobilization was optimized and the performance of the developed urea biosensor was evaluated. The obtained urea biosensor presented high-selectivity monitoring of urea, better reproducibility (S.D. = 0.02, n = 6), shorter response time (30 s), wider linear range (0.5 μM to 3 mM), lower detection limit (0.2 μM) and good long-term storage stability (with about 76% of the enzymatic activity retained after 30 days). The clinical analysis of the urea biosensor confirmed the feasibility of urea detection in urine samples. |
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Keywords: | Urea Activity Urease immobilization Urea biosensor Nanoporous alumina membranes |
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