The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase |
| |
Authors: | Wollman Yoram Blumberg Shmariahu Spungin Anya Brenneman Douglas E Fridkin Mati Wollman Jonathan Iaina Adrian Gozes Illana |
| |
Institution: | Department of Nephrology, Tel Aviv Medical Center, 64239, Tel Aviv, Israel. |
| |
Abstract: | Vasoactive intestinal peptide (VIP) stimulates the neuroblastoma cell line (NMB) to proliferate. Neuropeptide activity can be inhibited by neutral endopeptidases that function intracellularly and in the extracellular milieu. NMB cells express neutral endopeptidase (NEP) activity that can be specifically inhibited by phosphoramidon (PA). Our data now show that phosphoramidon treatment increases the efficacy of VIP-stimulated neuroblastoma proliferation. These results suggest that membrane endopeptidases modulate VIP-associated cell proliferation and enhancement of endopeptidase activity may serve as a target for cancer therapy. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |