Immunocytochemical localization of adrenodoxin in bovine adrenal cortex by protein A-gold technique |
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| Authors: | O Hatano Y Sagara T Omura A Takakusu |
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| Institution: | (1) Department of Anatomy, Nara Medical University, 840 Shijo-cho, 634 Kashihara, Nara, Japan;(2) Facilty of Pharmaceutical Science, Kyusyu University, 812 Fukuoka, Japan;(3) Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 812 Fukuoka, Japan |
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| Abstract: | Summary To investigate the intracellular localization of the enzymes that are involved in steroid hormone synthesis, an immunocytochemical study of the distribution of adrenodoxin in cells of the bovine adrenal cortex was carried out by the post-embedding immunostaining method and by immuno-cryoultramicrotomy in combination with the protein A-gold technique. Gold particles were seen on the matrix and the inner membrane of all the mitochondria examined, which have typical vesicular or tubulo-vesicular cristae, in parenchymal cells of the zona fasciculata and the zona reticularis. Gold particles were distributed homogeneously among the mitochondria. The density of gold particles on mitochondria in the parenchymal cells of the zona glomerulosa was lower than that of the zona fasciculata, which was similar to that of the zona reticularis. Gold particles were also seen on round, electron-dense intramitochondrial bodies in the parenchymal cells. The intramitochondrial bodies were abundant in the zona glomerulosa and the outer region of the zona fasciculata.Supported by a Grant-in-Aid for Scientific Research on Priority Areas (no. 63635505) from the Ministry of Education, Science and Culture of Japan |
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