| 应用RAPD标记检测导入普通小麦中的Elymus rectisetus遗传物质 |
| |
| 引用本文： | 鲁玉柱 薛秀庄 等. 应用RAPD标记检测导入普通小麦中的Elymus rectisetus遗传物质[J]. 西北植物学报, 2002, 22(1): 51-55 |
| |
| 作者姓名： | 鲁玉柱 薛秀庄 等 |
| |
| 作者单位： | 西北农林科技大学,陕西杨陵,712100 |
| |
| 基金项目： | 国家自然科学基金资助项目 ( 39870 5 2 9) |
| |
| 摘 要： | 以72个含16株系的BC2F5（小麦/Elymus rectisetus//小麦）单株为供体材料，用筛选出的12个10碱基随机引物对其进行多态性扩增。以E.rectisetus和Fukuhokomugi(Triticum aestivum)为亲本，建立RAPD标记。实验表明：12个随机引物中，有10个随机引物能够在16个株系的68个单株中分别扩增出普通小麦所没有的E.rectisetus的DNA片段，可分为6个类型，此外，1040-2-5-1和1048-Y3-3-1可能含5种异源染色体。
|
| 关 键 词： | 小麦 RAPD标记 披碱草遗传物质 检测 无融合生殖 |
| 文章编号： | 1000-4025(2002)01-0051-05 |
| 修稿时间： | 2001-01-12 |
Detection of Elymus rectisetus chromation transferred to wheat by RAPD markers |
| |
| LU Yu zhu,XUE Xiu zhuang,WANG Chang you,CHENG Yu quan. Detection of Elymus rectisetus chromation transferred to wheat by RAPD markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2002, 22(1): 51-55 |
| |
| Authors: | LU Yu zhu XUE Xiu zhuang WANG Chang you CHENG Yu quan |
| |
| Abstract: | To detect Elymus rectisetus chromation transferred to wheat, E.rectisetus ,Fukuhokomugi ( Triticum aestivum ) and 72 plants of 16 BC 2F 5 derivatives (wheat/ E.rectisetus //wheat) were used for RAPD analysis with 12 arbitrary primers (all operon 10 mer sequences).The results demonstrated that 10 out of 12 primers yielded E.rectisetus specific products among 68 plants of 16 BC 2F 5 derivatives.According to the results of RAPD analysis,six different type alien addition lines were identified among 68 plants.In addition,five different E.rectisetus chromosome were probably transferred to 1040 2 5 1 and 1048 Y 3 3 1 respectively. |
| |
| Keywords: | wheat Elymus rectisetus RAPD Marker |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北植物学报》浏览原始摘要信息 |
|
点击此处可从《西北植物学报》下载全文 |
