Influence of temperature on process efficiency and microbial community response during the biological removal of chlorophenols in a packed-bed bioreactor |
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Authors: | H Zilouei A Soares M Murto B Guieysse B Mattiasson |
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Institution: | (1) Department of Biotechnology, Lund University, P.O. Box 124, 22100 Lund, Sweden |
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Abstract: | Two reactors, initially operated at 14 and 23±1°C (RA and RB, respectively), were inoculated with a bacterial consortium enriched and acclimatized to the respective temperatures over 4 months. The biofilms, formed in the reactors, were studied using scanning electron microscopy, cultivation of the biofilm microflora, and physiological analysis of the isolates. Two bacteria able to mineralize chlorophenols under a large range of temperature (10–30°C) were isolated from the biofilm communities of reactors RA and RB and characterized as Alcaligenaceae bacterium R14C4 and Cupriavidus basilensis R25C6, respectively. When temperature was decreased by 10°C, the chlorophenols removal capacity was reduced from 51.6 to 22.8 mg l−1 h−1 in bioreactor RA (from 14 to 4°C) and from 59.3 to 34.7 mg l−1 h−1 in bioreactor RB (from 23±1 to 14°C). Fluorescence in situ hybridization (FISH) of the biofilm communities showed that, in all temperatures tested, the β-proteobacteria were the major bacterial community (35–47%) followed by the γ-proteobacteria (12.0–6.5%). When the temperature was decreased by 10°C, the proportions of γ-proteobacteria and Pseudomonas species increased significantly in both microbial communities. |
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