Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus |
| |
Authors: | Tanima Saha Debi Ghosh Srijata Mukherjee Shilpi Bose Mina Mukherjee |
| |
Institution: | aIndian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata 700032, India |
| |
Abstract: | Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88 U mL?1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate. CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30 °C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium. |
| |
Keywords: | Cellobiose dehydrogenase Termitomyces clypeatus Edible fungus Thermolabile enzyme Lactobionic acid Glucose effect |
本文献已被 ScienceDirect 等数据库收录! |