The use of FPLC for microdetermination of enzyme activity

Summary The FPLC system was used for the microdetermination of enzyme activities via the micromeasurement of absorbance of the reaction mixture. The sample loop was directly connected with the monitor. The solution to be measured was injected into the flow of water and the monitor response was measured as a peak height with the built-in integrator. Only 40 l of the solution are needed for one absorbance measurement.