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Type-3 copper proteins as biocompatible and reusable oxygen sensors |
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| Authors: | Gerhild Zauner Luigi Bubacco Armand W.J.W. Tepper |
| Affiliation: | a Leiden Institute of Chemistry, Leiden University, 2300 RA Leiden, The Netherlands b Department of Biology, University of Padua, Via U. Bassi 58B, 35121 Padua, Italy c Leiden Institute of Physics, Leiden University, 2300 RA, Leiden, The Netherlands |
| Abstract: | Fluorescently labeled type-3 copper proteins have been proposed previously as solution oxygen sensors by using a FRET mechanism. Herein, we describe how this principle can be adapted to sense O2 by means of proteins immobilized in optically transparent silica matrices. Specifically, the protein, hemocyanin from Octopus vulgaris N-terminally labeled with Cy5, is immobilized in two different kinds of optically transparent silica matrices, which appear to be a promising platform for enzyme encapsulation. The presented results provide proof of principle that fluorescently labeled proteins immobilized in a silica matrix can be implemented in a reusable, biocompatible and stable oxygen measuring device that might lead to new developments in the field of optical biosensing. |
| Keywords: | Sensitised fluorescence FRET Hemocyanin Sol-gel Oxygen sensor |
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