Involvement of G-alpha protein GNA3 in production of cell wall-degrading enzymes by Trichoderma reesei (Hypocrea jecorina) during mycoparasitism against Pythium ultimum |
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| Authors: | Roberto do Nascimento Silva Andrei Stecca Steindorff Cirano José Ulhoa Carlos Roberto Félix |
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| Institution: | (1) Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, 70910-900 Brasilia, DF, Brazil;(2) Laboratório de Enzimologia, Universidade Federal de Goiás (ICB), 74001-970 Goiania, GO, Brazil |
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| Abstract: | The involvement of the G-alpha protein GNA3 in the production of cell wall-degrading enzymes (CWDEs) by Trichoderma reesei during antagonism against Pythium ultimum was investigated. cAMP content was 2.8-fold higher in the T. reesei mutant gna3QL than in the parental TU-6. The gna3QL, like TU-6, inhibited the growth of P. ultimum in dual culture assays. Scanning electron microscopy showed that the gna3QL promoted more morphological alterations of P. ultimum cell wall than TU-6. In general, gna3QL produced higher activities of CWDEs than TU-6. We therefore suggest that CWDEs production
during mycoparasitism by T. reesei against P. ultimum may be associated with the level of GNA3 activity. |
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| Keywords: | Degrading enzymes G-protein Mycoparasitism Trichoderma reesei |
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