High-efficiency siRNA-based gene knockdown in human embryonic stem cells |
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Authors: | Ma Yinghong Jin Jianyu Dong Chunsheng Cheng Ee-Chun Lin Haifan Huang Yingqun Qiu Caihong |
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Institution: | Yale Stem Cell Center, New Haven, Connecticut 06520, USA. |
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Abstract: | Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown. |
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Keywords: | stem cells siRNA transfection |
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