A factor promoting the ejection of deacylated initiator transfer RNA from ribosomes

Experiments in bacterial cell-free extracts show that the deacylated initiator transfer RNA can be specifically discharged from its complex with the ribosome and messenger by addition of GTF and a factor derived from ribosomal salt-washings. This release-factor differs from that for the non-initiating tRNA's in its capability to dissociate the tRNA_f^Met-30 s ribosome-ApUpG complex^‡, in its inability to be inhibited by fusidic acid, and in its Chromatographic mobility on an ion-exchange column.